A Comprehensive Solution for E. coli Inclusion Bodies: From Ineffective Precipitation to an Industrial-Scale Process System for High-Activity Medicinal Proteins
In the field of industrial-scale expression of recombinant proteins in E. coli, inclusion body formation is a core, common challenge that limits product activity and hinders mass production. It is common during R&D for electrophoresis results to show excellent expression levels with bright bands, only for the protein to aggregate and become completely inactivated following purification.